TRIzol® 試劑是一種*的即用型試劑,可用于提取高質(zhì)量的總RNA或者從各種生物樣本中同時(shí)提取RNA、DNA和蛋白質(zhì)。這種酚和異硫氰酸胍單相溶液,可用于提取人、動(dòng)物、植物、酵母或細(xì)菌來源的細(xì)胞和組織樣本中的純RNA、DNA和蛋白質(zhì),只需一個(gè)小時(shí)即可完成。
• 從一份樣品同時(shí)提取RNA、DNA和蛋白質(zhì)
• 即便是難以處理的樣本類型,亦具有出色的裂解能力
• 優(yōu)化的配方和實(shí)驗(yàn)方案,適用于組織、細(xì)胞、血清、病毒和細(xì)菌等多種樣品類型
從多種樣本體積和來源獲得可靠的純化RNA
TRIzol® 試劑用于處理人、動(dòng)物、植物或細(xì)菌來源的少量組織 (50-100 mg) 和細(xì)胞 (5 × 106),以及大量組織 (≥1 g) 和細(xì)胞 (>107),均可獲得的性能,且附帶樣本純化實(shí)驗(yàn)方案。在TRIzol® 試劑進(jìn)行樣本勻漿化的過程中,它可以破壞細(xì)胞,溶解細(xì)胞組分,同時(shí)高效的抑制RNA酶的活性,從而維持RNA的完整性。TRIzol® 試劑方法十分簡(jiǎn)單,可同時(shí)處理大量樣本。整個(gè)過程可在一小時(shí)內(nèi)完成。采用TRIzol® 試劑提取的總RNA不會(huì)被蛋白質(zhì)和DNA污染。
適用于多個(gè)分子靶點(diǎn)的提取
TRIzol® 試劑使您可以順序沉淀單一樣本中的RNA、DNA和蛋白質(zhì)。采用TRIzol® 試劑進(jìn)行樣本勻漿化后,加入氯仿,勻漿物可分成透明的上層水相層 (含有RNA)、相界面和紅色的下層有機(jī)層 (含有DNA和蛋白質(zhì))。然后用異丙醇從水相層中沉淀出RNA。用從相界面/有機(jī)層中沉淀出DNA。利用異丙醇沉淀從酚-上清液中沉淀出蛋白質(zhì)。洗滌沉淀的RNA、DNA或蛋白質(zhì),去除雜質(zhì),重懸浮后供下游應(yīng)用。
僅供研究使用。不得用于動(dòng)物或人類的診斷或治療。
詳細(xì)說明
常用規(guī)格
Shipping Condition: | Room Temperature |
Sample Type (General): | Viral Samples, Tissue, Blood, Bacteria, Yeast, Cells, Plant Samples |
Final Product: | Transcriptome RNA, micro RNA, Total RNA |
Downstream Application: | Cloning, Real-Time PCR, cDNA Library Construction, Nuclease Protection Assays, Northern Blotting, RT-PCR (Endpoint) |
High Throughput Compatibility: | Not High Throughput-Compatible, Manual Protocols |
Isolation Technology: | Organic Extraction |
Scale: | Scaleable |
System: | TRIzol |
Quantity: | 1 |
Product Size: | 100 ml |
Purification Time: | 1 hr |
Number of Reactions: | 100 Preps |
Starting Material (Amount): | Up to 1 g tissue, Up to 1 x 10^7 cells |
Regulatory Statement: | For Research Use Only. Not for use in diagnostic procedures. |
(以下為本公司常備原裝現(xiàn)貨)
invitrogen 原裝產(chǎn)品
品牌 | 貨號(hào) | 名稱 | 規(guī)格 |
invitrogen | 13778-075 | Lipofectamine RNAiMAX | 0.75ml |
invitrogen | 13778-150 | Lipofectamine RNAiMAX | 1.5ml |
invitrogen | 11668-027 | Lipofectamine 2000 Reagent | 0.75ml |
invitrogen | 11668-019 | Lipofectamine 2000 Reagent | 1.5ml |
invitrogen | 15596-026 | TRlzol ®Reagent | 100ml |
invitrogen | 15596-018 | TRlzol ®Reagent | 200ml |
invitrogen | 10296-010 | Trizol ® LS Reagent | 100ml |
invitrogen | 10296-028 | Trizol ® LS Reagent | 200ml |
invitrogen | 17504-044 | B-27(黃顏色) | 10ml |
invitrogen | 12587-010 | B-27(黃顏色)不含維生素A | 10ml |
invitrogen | 17502-048 | N-2 (粉紅顏色) | 5ml |
invitrogen | 18064-014 | SuperScript® II Reverse Transcriptase | 10,000U |
invitrogen | 18080-044 | SuperScript® III Reverse Transcriptase | 10,000U |
第二部分 HyClone胎牛血清
品牌 | 貨號(hào) | 名稱 | 規(guī)格 |
HyClone | SH30370.03 | Australian Standard Fetal Bovine Serum | 500ml |
HyClone | SH30406.02 | FOETAL BOVINE SERUM | 500ml |
HyClone | SH30401.01 | New Zealand Calf Serum | 500ml |
HyClone | SH30066.02 | FetalClone® II | 100ml |
HyClone | SH30088.03 | STANDARD FETAL BOVINE SERUM | 500ml |
HyClone | SH30118.02 | Newborn Bovine Calf Serum, U.S. Origin | 100ml |
HyClone | SH30070.02 | FETAL BOVINE SERUM | 100ml |
HyClone | SH30087.02 | COSMIC CALF® SERUM | 100ml |
HyClone | SH30070.03E | FETALBOVINESERUM CHARACTERIZSED | 500ml |
100ml | |||
HyClone | SH30070.03 | FETAL BOVINE SERUM | 500ml |
HyClone | SH30080.03 | FetalClone® I | 500ml |
HyClone | SH30084.03 | FOETAL BOVINE SERUM: CERTIFIED AUSTRALIAN | 500ml |
HyClone | SH30396.03 | FETAL BOVINE SERUM CHARACTERIZED | 500ml |
HyClone | SV30087.02 | 南美源胎牛血清 | 500ml |
HyClone | sv30087.01 | 南美源胎牛血清 | 100ml |
HyClone | SH30071.03 | FETAL BOVINE SERUM | 500ml |
HyClone | SH30071.02 | FETAL BOVINE SERUM | 100ml |
HyClone | SH30080.02 | FetalClone® I | 100ml |
HyClone | SH30070.02E | FETAL BOVINE SERUM CHARACTERIZSED | 100ml |
HyClone | SH30079.02 | FETAL BOVINE SERUM | 100ml |
HyClone | SH30068.02 | CHARCOAL/DEXTRAN TREATED FBS, 活性炭滅活血清 | 100ml |
HyClone | SH30068.03 | CHARCOAL/DEXTRAN TREATED FBS, 活性炭滅活血清 | 500ml |
HyClone | SH30066.03 | FetalClone® II | 500ml |
HyClone | SH30109.03 | HyClone FetalClone® III | 500ml |
HyClone | SH30088.03 | STANDARD FETAL BOVINE SERUM | 500ml |
HyClone | SH30088.02 | STANDARD FETAL BOVINE SERUM | 100ml |
HyClone | SH30079.03 | FETAL BOVINE SERUM | 500ml |
第二部分 Gibco胎牛血清
品牌 | 貨號(hào) | 名稱 | 規(guī)格 |
Gibco | 12662-029 | MSC QUALIFIED FBS 500ML | 500ML |
Gibco | 10438-026 | FBS, HI 500ML | 500ML |
Gibco | 10439-024 | FBS, ES CELL QUAL. USDA APPD. 500ML | 500ML |
Gibco | 10099-141 | FBS, AUSTRALIAN ORIGIN 500ML | 500ML |
Gibco | 26400-044 | FBS, DIALYZED, USA ORIGIN 500ML | 500ML |
Gibco | 26140-079 | FBS QUALIFIED, USA ORIGIN 500ML | 500ML |
Gibco | 16141-079 | FBS, ES CELL QUAL, USA ORIGIN 500ML | 500ML |
Gibco | 16140-071 | FBS, HI 500ML | 500ML |
Gibco | 16000-044 | FBS, CERT, USA ORIGIN 500ML | 500ML |
Gibco | C2027050 | FBS, URUGUAYAN ORIGIN | 500ML |
Gibco | 12657-029 | 巴西胎牛 | 500ml |