• Concentrates 500 µL down to 15 µL
   
• Concentration factor 25 x to 30 x
   
• High sample recovery （greater than 90% of dilute starting solution）
   
• Fast processing time - 10 to 30 minutes
   
• Consistent recoveries thanks to the reverse spin capability
   
• Reverse spin capability provides consistent recoveries

Applications

• Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
   
• Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromelecular labels from a reaction mix, and protein removal prior to HPLC
   
• Desalting, buffer exchange, and protein dialysis

Performance – DNA Concentration

The Amicon Ultra-0.5 device provides the best balance between recovery and spin time for double-stranded DNA for base pairs ranging from 137 to 1159. To achieve maximum PCR product recovery and primer removal with primers greater than 20 bases, one or two additional spins with Tris-EDTA （TE） buffer are recommended.

Desalting or Diafiltration

Desalting, buffer exchange or diafiltration are important methods for removing salts or solvents in solutions containing biomolecules. The removal of salts or the exchange of buffers can be accomplished in the Amicon Ultra-0.5 device by concentrating the sample, then reconstituting the concentrate to the original sample volume with any desired solvent. The process of "washing out" can be repeated until the concentration of the contaminating microsolute has been sufficiently reduced. See example below.