移動式葉綠素熒光成像系統(tǒng)PlantExplorer^XS是由慧諾瑞德和荷蘭PhenoVation公司聯(lián)合推出的專門針對大田、溫室、氣候室和實驗室場景的可以移動的葉綠素熒光測量系統(tǒng)。配備移動式升降平臺車、內置電腦的葉綠素熒光成像單元、移動電源、顯示單元和操作單元。葉綠素熒光成像單元可以升降和旋轉，既可以測量不同高度的植物冠層，也可以傾斜或水平角度測量穗（麥穗、稻穗、谷穗等）、莢果（大豆、油菜等）、果實（番茄、黃瓜、葡萄、柑橘等）、葉片或冠層。

該系統(tǒng)成像面積為18x18cm，具備500萬像素高清成像，同時具備“調制”和“非調制”葉綠素熒光成像測量功能，既可以測量光合生理，也可以測量形態(tài)結構，同時配備功能強大的控制和分析軟件，且可以對大量數(shù)據(jù)進行批處理分析。該系統(tǒng)，無論室內還是大田，都是進行植物表型、光合生理、植物抗逆、植物病理、育種、功能基因組、突變株篩選、種子生理/病理等研究的利器。
 

功能特性

• 大田、溫室、氣候室、實驗室進行移動式測量
• 葉綠素熒光成像單元可以升降、旋轉
• 葉綠素熒光成像和表型分析同步測量
• 同時具備調制和非調制葉綠素熒光測量功能
• 出色的高清相機(500萬像素)、高信噪比成像
• 16位圖像格式，的成像質量
• 光源、相機、濾光片、電腦一體化設計
• 無可見鏡頭畸變，無需圖像校正
• 成像范圍18 x 18cm
• 多種測量protocol可選，允許用戶編輯設定自己的protocol，包括但不限于Fv/Fm測量、標準誘導曲線測量、暗弛豫測量、OJIP快速誘導動力學測量等等。
• 可進行功能強大的延時成像測量
• 自動計算熒光參數(shù)和表型參數(shù)
• 具備圖像數(shù)據(jù)批處理分析功能
• 提供多種功能強大的圖像分割功能
• 對所有圖像數(shù)據(jù)均提供數(shù)據(jù)分級(用戶自定義范圍)并進行圖像化顯示，并允許對分級篩選后的數(shù)據(jù)疊加到可見光圖像上展示
• 圖像背景、偽彩色標尺均有多種選擇
• 允許用戶自定義多種ROI(性狀、數(shù)目、分布等)并對ROI的數(shù)據(jù)自動分析
• 嵌入式電腦進行精確的成像、時間控制、光強控制和數(shù)據(jù)存儲
• 功能強大的控制和分析軟件
• 特別適合突變株篩選、育種材料/組合篩選、抗逆研究、病理研究、種子研究、果實研究、功能基因組學等

主要技術參數(shù)

• 基本組成：移動式升降平臺、葉綠素熒光成像單元、移動電源、顯示單元、操作單元等
• 葉綠素熒光成像方式：“調制”測量 +“費調制”測量
• 調制測量光：藍色LED， 450nm，半峰全寬20nm，光強4000 umol m^-2 s^-1 ，獨立觸發(fā)
• Kautsky測量光：藍色LED， 450nm，半峰全寬20nm，光強4000 umol m^-2 s^-1
• 飽和脈沖：藍色LED， 450nm，半峰全寬20nm，光強4000 umol m^-2 s^-1，獨立觸發(fā)
• 時間分辨動力學光化光：紅光LED，660nm，光強800 umol m^-2 s^-1
• 遠紅光：LED，735nm，半峰全寬20nm，35W
• 相機：CMOS傳感器，500萬像素
• 顏色深度：12bit
• 標準幀率：37.5 FPS
• 圖像格式：16bit
• 相機光譜范圍：400～1000 nm
• 接口：3個USB3.0，1個以太網(wǎng)口，1個HDMI接口
• 嵌入式電腦：4核處理器，8G內存，256G固態(tài)硬盤
• 成像面積：18cm x 18cm
• 升降高度：0-1200mm（高度可定制）
• 旋轉角度：-90° ~ 90°
• 顯示單元：15.6寸觸摸顯示屏
• 供電：35萬mAh移動電源，額定容量1260Wh，峰值功耗1000W，待機功耗35W
• 系統(tǒng)尺寸：600mm x 720mm x 2000mm(長x寬x高）

測量參數(shù)

• 調制葉綠素熒光參數(shù)：Fo、Fm、Fv/Fm、dFq/Fm=DF/Fm、Fs’、Fm’、Fo’、Fq’/Fm’=Fv’/Fm’、rETR、NPQ、Y(NO)、Y(NPQ)、qN、qP、qL、1-qP和1-qL等;
• 非調制葉綠素熒光參數(shù)：Fo、Fi、Fm、1-Fi/Fm、IC-Area、IC-Area/Fv、PI、Rfd、dRfd、RfdFm和RfdFt等;
• 表型參數(shù)：(植物、種子、果實的)數(shù)目、輪廓面積、長度、寬度、凸包點數(shù)、凸包面積、凸包面積/輪廓面積、最小外接圓(質心、半徑、面積)、最小外接矩形(長、寬、面積、角度、alpha)和骨架等。

利用PhenoVation葉綠素熒光成像技術發(fā)表的部分文獻

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