aimbiotech 3D Cell Culture Chip,三維微流控芯片系統(tǒng)現(xiàn)貨

• 1、AIM Biotech 3D細(xì)胞培養(yǎng)芯片采用三通道設(shè)計，中間為3D凝膠通道，兩側(cè)為培養(yǎng)基通道，通過負(fù)壓吸引快速交換培養(yǎng)基。
  2、芯片透氣性好，可有效進(jìn)行氣體交換 3采用標(biāo)準(zhǔn)載玻片尺寸（75 mm x 25 mm），兼容相差顯微鏡、熒光顯微鏡和激光共聚焦顯微鏡 4可實現(xiàn)不同類型細(xì)胞共培養(yǎng)。
   

  AIM BIOTECH是新加坡一家專注于創(chuàng)新性工具研發(fā)的創(chuàng)業(yè)型公司,其應(yīng)用領(lǐng)域涵蓋科學(xué)研究、藥物開發(fā)和臨床診斷范疇。AIM BIOTEC為科研市場做出的份貢獻(xiàn)是開發(fā)出一款易于操作的、模塊化的平臺，該平臺成功地將3D細(xì)胞培養(yǎng)納入了科研人員研究工作體系之中。
  AIM BIOTECH 3D細(xì)胞培養(yǎng)芯片概述
  AIM的3D細(xì)胞培養(yǎng)芯片透氣性好，而且用戶可以通過選擇不同的水凝膠，在間隔的3D和2D空間進(jìn)行不同類型細(xì)胞的培養(yǎng)。同時可以通過對化學(xué)物濃度梯度和流體的調(diào)控很好地模擬符合用戶te定需求的微環(huán)境。

• 訂貨信息（備有）：

  1、3D Cell Culture Chips DAX-1 (25/box)

  

  2、Holders HOL-1 (10/box)

  

  3、Luer Connectors LUC-1 (36/pack)

  
  
  
  
  
  
  

   

  3D Cell Culture Chip 3-channel design : 3D gel region flanked by 2 media channels Microscope slide format 75mm X 25mm Compatible with all polymerisable gels including collagen, fibrinogen, Matrigel, etc. and combinations thereof Gas permeable laminate for effective gas exchange Optically clear and compatible with phase contrast, fluorescence and confocal microscopy Enables monotypic or organotypic co-culture models Enables the control of interstitial flow across the 3D gel region Enables the control of chemical gradients across the 3D gel region Sterile & ready-to-use Designed for rapid media exchange through vacuum aspiration with no risk of over-aspiration Designed for modular expansion with AIM Luer Connectors Fits into AIM Microtiter Plate Holders for easy handling and stacking GENERAL PROTOCOLS APPLICATION-SPECIFIC PROTOCOLS

  BUY NOW

  Compatible with all polymerisable gels Dedicated 3D regions in AIM chips can be filled with collagen, fibrinogen & other hydrogels or Matrigel? & other extracellular matrixes (ECM) to suit your experimental needs. The hydrogels can be used on their own or in combination with other components to form 3D microenvironments of your choice (stiffness, pH and material compositions).  The miniature posts that border the 3D region are designed to set up a vertical gel wall with minimal buildup of resistance during the gel filling process. Cells can be homogeneously dispersed or included as aggregates into the gel.

  Gas exchange One of the key advantages of PDMS chips is the material's gas permeability, which enables cells cultured within PDMS devices to 'breathe'. However, PDMS absorbs hydrophobic molecules from solution, making it unsuitable for studies investigating hydrophobic drugs, chemicals or biological molecules. AIM chips have overcome the problem by using a gas-permeable plastic to laminate the device. Gas exchange takes place effectively, allowing you to set up normoxic or hypoxic culture environments as needed.

  Optically clear AIM chips are made from polymers with an excellent light transmittance rate of 92%. You can visualise your experiments with phase contrast, epifluorescence, 2-photon and confocal microscopy.

  Endothelial cell monolayer in 2D channel forming a vertical wall on collagen gel (confocal)

  Angiogenic sprouts in collagen gel (confocal)

  Enables monotypic or organotypic co-culture models Different cell types can be cultured together in the same channel or compartmentalised into different channels, allowing users to design models to represent different biological systems. Future AIM chips will have more 3D & 2D channel designs to cater to your needs.

  Enables the control of interstitial flow across the 3D region The interstitial flow across the 3D hydrogel can be controlled by setting up a pressure gradient between the flanking channels. This can be achieved by having a larger media volume in one media channel than the other, or by setting shear flow regimes that establish a pressure differential.

  Enables the control of chemical gradients across the 3D region A chemical concentration gradient can be set up across the porous 3D hydrogel easily by using a higher concentration of the chemical in a channel and allowing diffusion to take place.  This feature is very useful for studies where directional cues of effectors are critical, including angiogenesis, cell migration and neurite guidance

  Sterile & ready-to-use AIM chips are individually packaged for your convenience. All chips are sterile and are ready for use right out of the package. AIM chips let you focus on your experiments, rather than on device preparation.

  Designed for rapid media exchange through vacuum aspiration with no risk of over-aspiration Due to the small culture volumes of microfluidic devices, culture media typically has to be replenished every day. Vacuum aspiration is used to remove old media before pipetting new media into the device. Media ports in AIM chips are designed with troughs to let users rapidly aspirate old media out without the risk of accidentally aspirating all the media & cells from the device.    Publications Many of the publications listed below were conducted on lab-made devices that form the basis of AIM Biotech chips. Papers that employed the commercial chips are marked with '*'. TECHNOLOGY Key Publications Design, fabrication and implementation of a novel multi-parameter control microfluidic platform for three-dimensional cell culture and real-time imaging. Vickerman V, Blundo J, Chung S, Kamm RD. Lab Chip, 2008, 8, 1468-1477. Cell migration into scaffold under co-culture conditions in a microfluidic platform. Chung S, Sudo S, Mack PJ, Wan C-R, Vickerman V, Kamm RD. Lab Chip, 2009, 9(2):269-75. Microfluidic assay for simultaneous culture of multiple cell types on surfaces or within hydrogels. Shin Y, Han S, Jeon JS, Yamamoto K, Zervantonakis IK, Sudo R, Kamm RD and Chung S. Nature Prot, 7(7):1247-1259, 2012, PMID: Mechanism of a flow-gated angiogenesis switch: early signaling events at cell-matrix and cell-cell junctions. Vickerman V, Kamm RD. Integr Biol (Camb). 2012 Jun 7. PMID Three-dimensional microfluidic model for tumor cell intravasation and endothelial barrier function. Zervantonakis IK, Hughes-Alford SK, Charest JL, Condeelis JS, Gertler FB, Kamm RD. Proc Natl Acad Sci U S A. 2012 Aug 21;109(34):13515-20. Epub 2012 Aug 6. PMID: Screening therapeutic EMT blocking agents in a three-dimensional microenvironment. Aref AR, Huang RY-J, Yu W, Chua K-N, Sun W, Tu T-Y, Sim W-J, Zervantonakis IK, Thiery JP, Kamm RD. Integr Biol (Camb). 2013 Feb;5(2):381-9. doi: 10.1039/c2ib20209c PMID: Mechanotransduction of fluid stresses governs 3D rheotaxis. Polacheck WJ, German AE, Mammoto A, Ingber DE, Kamm RD. Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2447-52. doi: 10.1073/pnas.1316848111. Epub 2014 Feb 3. PMID: Human 3D vascularized organotypic microfluidic assays to study breast cancer cell extravasation. Jeon JS, Bersini S, Gilardi M, Dubini G, Charest JL, Moretti M, Kamm RD. Proceedings of the National Academy of Sciences, pp. 201417115, 2014 *Ex Vivo Profiling of PD-1 Blockade Using Organotypic Tumor Spheroids. Jenkins RW, Aref AR, Lizotte PH, Ivanova E, Stinson S, Zhou CW, ... Barbie DA. Cancer Discov. 2017 Nov 3. pii: CD-17-0833. doi: 10.1158/2159-8290.CD-17-0833. 3D self-organized microvascular model of the human blood-brain barrier with endothelial cells, pericytes and astrocytes. Campisi M, Shin YJ, Osaki T, Hajal C, Chiono V, Kamm RD. Biomaterials 2018 https://doi.org/10.1016/j.biomaterials.2018.07.014 Latest Publications *MBNL1 alternative splicing isoforms play opposing roles in cancer. Tabaglio T, Low DHP, Teo WKL, Goy PA, Cywoniuk P, Wollmann H... Guccione E. Life Science Alliance, Sept 2018 doi:10.26508/lsa.201800157 *3D microfluidic ex vivo culture of organotypic tumor spheroids to model immune checkpoint blockade. Aref AR, Campisi M, Ivanova E, Portell A, Larios D, Piel BP... Jenkins RW. Lab on a Chip, 2018, DOI: 10.1039/C8LC00322J *Molecular recalibration of PD-1+ antigen-specific T cells from blood and liver. Otano I, Escors D, Schurich A, Singh H, Robertson F, Davidson BR... Maini MK. Molecular Therapy (2018), doi: 10.1016/j.ymthe.2018.08.013. *Tumor innate immunity primed by specific interferon-stimulated endogenous retroviruses. Ca?adas I, Thummalapalli R, Kim JW, Kitajima S, Jenkins RW, Christensen CL... Barbie DA. Nature Medicine 23 July 2018 doi.org/10.1038/s41591-018-0116-5 3D self-organized microvascular model of the human blood-brain barrier with endothelial cells, pericytes and astrocytes. Campisi M, Shin YJ, Osaki T, Hajal C, Chiono V, Kamm RD. Biomaterials 2018 https://doi.org/10.1016/j.biomaterials.2018.07.014 *Assessing Therapeutic Efficacy of MEK Inhibition in a KRASG12C-Driven Mouse Model of Lung Cancer. Li S, Liu S, Deng J, Akbay EA, Hai J, Ambrogio C ... Wong KK. Clinical Cancer Research 2018 doi: 10.1158/1078-0432.CCR-17-3438? Characterizing the Role of Monocytes in T Cell Cancer Immunotherapy Using a 3D Microfluidic Model. Lee SWL, Adriani G, Ceccarello E, Pavesi A, Tan AT, Bertoletti A, Kamm RD and Wong SC (2018) Front. Immunol. 9:416. doi: 10.3389/ mmu.2018.00416 *Stimuli-Responsive Nanodiamond-Based Biosensor for Enhanced Metastatic Tumor Site Detection. Wang X, Gu MJ, Toh TB, Abdullah NLB, Chow E. SLAS Technol. 2018 Feb;23(1):44-56. doi: 10.1177/2472630317735497. Epub 2017 Oct 11.? *Protein corona of airborne nanoscale PM2.5 induces aberrant proliferation of human lung fibroblasts based on a 3D organotypic culture. Li Y, Wang PC, Hu CL, Wang K, Chang Q, Liu LJ, Han ZG, Shao Y, Zhai Y, Zuo ZY, Gong ZY, Wu Y. Scientific Reports volume 8, Article number: 1939(2018) doi:10.1038/s41598-018-20445-7 *Functional human 3D microvascular networks on a chip to study the procoagulant effects of ambient fine particulate matter. Li Y, Pi QM, Wang PC, Liu LJ, Han ZG, Shao Y, Zhai Y, Zuo ZY, Gong ZY, Yang X, Yang W. RSC Adv., 2017, 7, 56108–56116 *Ex Vivo Profiling of PD-1 Blockade Using Organotypic Tumor Spheroids. Jenkins RW, Aref AR, Lizotte PH, Ivanova E, Stinson S, Zhou CW, ... Barbie DA. Cancer Discov. 2017 Nov 3. pii: CD-17-0833. doi: 10.1158/2159-8290.CD-17-0833. *CDK4/6 Inhibition Augments Anti-Tumor Immunity by Enhancing T Cell Activation. Deng J, Wang ES, Jenkins RW, Li S, Dries R, Yates K, ... Wong KK. Cancer Discov. 2017 Nov 3. pii: CD-17-0915. doi: 10.1158/2159-8290.CD-17-0915.? *?A 3D microfluidic model for preclinical evaluation of TCR-engineered T cells against solid tumors. Pavesi A, Tan AT, Koh S, Chia A, Colombo M, Antonecchia E, Miccolis C, Ceccarello E, Adriani G, Raimondi MT, Kamm RD, Bertoletti A. JCI Insight. 2017 Jun 15;2(12). pii: 89762. doi: 10.1172/jci.insight.89762. VASCULAR FUNCTIONS CANCER BIOLOGY IMMUNOTHERAPY NEUROBIOLOGY STEM CELL BIOLOGY MECHANOBIOLOGY OTHER MODELS REVIEWS 1. Vascular Functions 1.1. Angiogenesis Design, fabrication and implementation of a novel multi-parameter control microfluidic platform for three-dimensional cell culture and real-time imaging. Vickerman V, Blundo J, Chung S, Kamm R. Lab Chip, 2008. 8 (9):1468-1477 Surface-Treatment-Induced Three-Dimensional Capillary Morphogenesis in a Microfluidic Platform. Chung S, Sudo R, Zervantonakis IK, Rimchala T, Kamm RD. Advanced Materials, 2009. 21 (47):4863-4867 Transport-mediated angiogenesis in 3D epithelial coculture. Sudo R, Chung S, Zervantonakis IK, Vickerman V, Toshimitsu Y, Griffith LG, Kamm RD. FASEB J., 2009. 23 (7):2155-2164 Determining Cell Fate Transition Probabilities to VEGF/Ang 1 Levels: Relating Computational Modeling to Microfluidic Angiogenesis Studies. Das A, Lauffenburger D, Asada H, Kamm R. Cellular and Molecular Bioengineering, 2010. 3 (4):345-360 Sprouting angiogenesis under a chemical gradient regulated by interactions with an endothelial monolayer in a microfluidic platform. Jeong GS, Han S, Shin Y, Kwon GH, Kamm RD, Lee SH, Chung S. Analytical Chemistry, 2011. 83 (22):8454-8459 In vitro 3D collective sprouting angiogenesis under orchestrated ANG-1 and VEGF gradients. Shin Y, Jeon JS, Han S, Jung GS, Shin S, Lee SH, . . . Chung S. Lab Chip, 2011. 11 (13):2175-2181 Ensemble Analysis of Angiogenic Growth in Three-Dimensional Microfluidic Cell Cultures. Farahat WA, Wood LB, Zervantonakis IK, Schor A, Ong S, Neal D, . . . Asada HH. PLoS ONE, 2012. 7 (5):e37333 Engineering of In Vitro 3D Capillary Beds by Self-Directed Angiogenic Sprouting. Chan JM, Zervantonakis IK, Rimchala T, Polacheck WJ, Whisler J, Kamm RD. PLoS ONE, 2012. 7 (12):e50582 Microfluidic assay for simultaneous culture of multiple cell types on surfaces or within hydrogels. Shin Y, Han S, Jeon JS, Yamamoto K, Zervantonakis IK, Sudo R, . . . Chung S. Nature Protocols, 2012. 7 (7):1247-1259 In vitro angiogenesis assay for the study of cell-encapsulation therapy. Kim C, Chung S, Yuchun L, Kim M-C, Chan JKY, Asada HH, Kamm RD. Lab Chip, 2012. 12 (16):2942-2950 Complementary effects of ciclopirox olamine, a prolyl hydroxylase inhibitor and sphingosine 1-phosphate on fibroblasts and endothelial cells in driving capillary sprouting. Lim SH, Kim C, Aref AR, Kamm RD, Raghunath M. Integr. Biol., 2013. 5 (12):1474-1484 ? 1.2. Anti-Angiogenesis The stabilization effect of mesenchymal stem cells on the formation of microvascular networks in a microfluidic device. Yamamoto K, Tanimura K, Mabuchi Y, Matsuzaki Y, Chung S, Kamm RD, . . . Sudo R. J. Biomech. Sci. Eng., 2013. 8 (2):114-128 Dll4-containing exosomes induce capillary sprout retraction in a 3D microenvironment. Sharghi-Namini S, Tan E, Ong L-LS, Ge R, Asada HH. Sci. Rep., 2014. 4:4031 A quantitative microfluidic angiogenesis screen for studying anti-angiogenic therapeutic drugs. Kim C, Kasuya J, Jeon J, Chung S, Kamm RD. Lab Chip, 2015. 15 (1):301-310 ? 1.3. Vasculogenesis Control of Perfusable Microvascular Network Morphology Using a Multiculture Microfluidic System. Whisler JA, Chen MB, Kamm RD. Tissue Engineering Part C: Methods, 2014. 20 (7):543-552 In Vitro Microvessel Growth and Remodeling within a Three-Dimensional Microfluidic Environment. Park Y, Tu T-Y, Lim S, Clement IM, Yang S, Kamm R. Cellular and Molecular Bioengineering, 2014. 7 (1):15-25 Generation of 3D functional microvascular networks with human mesenchymal stem cells in microfluidic systems. Jeon JS, Bersini S, Whisler JA, Chen MB, Dubini G, Charest JL, . . . Kamm RD. Integr. Biol., 2014. 6 (5):555-563 Human Vascular Tissue Models Formed from Human Induced Pluripotent Stem Cell Derived Endothelial Cells. Belair DG, Whisler JA, Valdez J, Velazquez J, Molenda JA, Vickerman V, . . . Murphy WL. Stem Cell Reviews and Reports, 2015. 11 (3):511-525 Elucidation of the Roles of Tumor Integrin β1 in the Extravasation Stage of the Metastasis Cascade. Chen MB, Lamar JM, Li R, Hynes RO, Kamm RD. Cancer Res., 2016. 76 (9):2513-2524 On-chip human microvasculature assay for visualization and quantitation of tumor cell extravasation dynamics. Chen MB, Whisler JA, Fr?se J, Yu C, Shin YJ and Kamm RD. Nat Protoc. 2017 May; 12(5): 865–880. *Functional human 3D microvascular networks on a chip to study the procoagulant effects of ambient fine particulate matter. Li Y, Pi QM, Wang PC, Liu LJ, Han ZG, Shao Y, Zhai Y, Zuo ZY, Gong ZY, Yang X, Yang W. RSC Adv., 2017, 7, 56108–56116 ? 1.4. Flow Response Mechanism of a flow-gated angiogenesis switch: Early signaling events at cell-matrix and cell-cell junctions. Vickerman V, Kamm RD. Integr. Biol., 2012. 4 (8):863-874 ? 1.5. Transendothelial Migration A versatile assay for monitoring in vivo-like transendothelial migration of neutrophils. Han S, Yan JJ, Shin Y, Jeon JJ, Won J, Jeong HE, . . . Chung S. Lab Chip, 2012. 12 (20):3861-3865 ? 1.6. Migration Vascular Endothelial Growth Factor (VEGF) and Platelet (PF-4) Factor 4 Inputs Modulate Human Microvascular Endothelial Signaling in a Three-Dimensional Matrix Migration Context. Hang T-C, Tedford NC, Reddy RJ, Rimchala T, Wells A, White FM, . . . Lauffenburger DA. Molecular & Cellular Proteomics : MCP, 2013. 12 (12):3704-3718 Cell Invasion Dynamics into a Three Dimensional Extracellular Matrix Fibre Network. Kim M-C, Whisler J, Silberberg YR, Kamm RD, Asada HH. PLoS Comput Biol, 2015. 11 (10):e1004535 ? 1.7. Permeability Constructive remodeling of a synthetic endothelial extracellular matrix. Han S, Shin Y, Jeong HE, Jeon JS, Kamm RD, Huh D, . . . Chung S. Sci. Rep., 2015. 5:18290 ↑ Back to the top 2. Cancer Biology 2.1. Spheroid Dispersion Screening therapeutic EMT blocking agents in a three-dimensional microenvironment. Aref AR, Huang RY-J, Yu W, Chua K-N, Sun W, Tu T-Y, . . . Kamm RD. Integr. Biol., 2013. 5 (2):381-389 Validating Antimetastatic Effects of Natural Products in an Engineered Microfluidic Platform Mimicking Tumor Microenvironment. Niu Y, Bai J, Kamm RD, Wang Y, Wang C. Mol. Pharm., 2014. 11 (7):2022-2029 Inhibition of KRAS-driven tumorigenicity by interruption of an autocrine cytokine circuit. Zhu Z, Aref AR, Cohoon TJ, Barbie TU, Imamura Y, Yang S, . . . Barbie DA. Cancer Discov., 2014. 4 (4):452-465 Targeting an IKBKE cytokine network impairs triple-negative breast cancer growth. Barbie TU, Alexe G, Aref AR, Li S, Zhu Z, Zhang X, . . . Gillanders WE. The Journal of Clinical Investigation, 2014. 124 (12):5411-5423 Development of covalent inhibitors that can overcome resistance to first-generation FGFR kinase inhibitors. Tan L, Wang J, Tanizaki J, Huang Z, Aref AR, Rusan M, . . . Gray NS. Proc. Natl. Acad. Sci. USA, 2014. 111 (45):E4869-E4877 Identification of drugs as single agents or in combination to prevent carcinoma dissemination in a microfluidic 3D environment. Bai J, Tu T-Y, Kim C, Thiery JP, Kamm RD. Oncotarget, 2015. 6 (34):36603-36614 Contact-dependent carcinoma aggregate dispersion by M2a macrophages via ICAM-1 and β2 integrin interactions. Bai J, Adriani G, Dang T-M, Tu T-Y, Penny H-XL, Wong S-C, . . . Thiery J-P. Oncotarget, 2015. 6 (28):25295-25307 *Stimuli-Responsive Nanodiamond-Based Biosensor for Enhanced Metastatic Tumor Site Detection. Wang X, Gu MJ, Toh TB, Abdullah NLB, Chow E. SLAS Technol. 2018 Feb;23(1):44-56. doi: 10.1177/2472630317735497. Epub 2017 Oct 11. ? 2.2. Extravasation In Vitro Model of Tumor Cell Extravasation. Jeon JS, Zervantonakis IK, Chung S, Kamm RD, Charest JL. PLoS ONE, 2013. 8 (2):e56910 Mechanisms of tumor cell extravasation in an in vitro microvascular network platform. Chen MB, Whisler JA, Jeon JS, Kamm RD. Integr. Biol., 2013. 5 (10):1262-1271 A microfluidic 3D in vitro model for specificity of breast cancer metastasis to bone. Bersini S, Jeon JS, Dubini G, Arrigoni C, Chung S, Charest JL, . . . Kamm RD. Biomaterials, 2014. 35 (8):2454-2461 Human 3D vascularized organotypic microfluidic assays to study breast cancer cell extravasation. Jeon JS, Bersini S, Gilardi M, Dubini G, Charest JL, Moretti M, Kamm RD. Proc. Natl. Acad. Sci. USA, 2015. 112 (1):214-219 Neutrophils suppress intraluminal NK-mediated tumor cell clearance and enhance extravasation of disseminated carcinoma cells. Spiegel A, Brooks MW, Houshyar S, Reinhardt F, Ardolino M, Fessler E, . . . Weinberg RA. Cancer Discov., 2016. 6 (6):630-649 Warburg metabolism in tumor-conditioned macrophages promotes metastasis in human pancreatic ductal adenocarcinoma. Penny HL, Sieow JL, Adriani G, Yeap WH, See Chi Ee P, San Luis B, . . . Wong SC. OncoImmunology, 2016. 5 (8):e1191731 ?On-chip human microvasculature assay for visualization and quantitation of tumor cell extravasation dynamics. Chen MB, Whisler JA, Fr?se J, Yu C, Shin YJ and Kamm RD. Nat Protoc. 2017 May; 12(5): 865–880. ? 2.3. Intravasation Three-dimensional microfluidic model for tumor cell intravasation and endothelial barrier function. Zervantonakis IK, Hughes-Alford SK, Charest JL, Condeelis JS, Gertler FB, Kamm RD. Proc. Natl. Acad. Sci. USA, 2012. 109 (34):13515-13520 ? 2.4. Flow Response Interstitial flow influences direction of tumor cell migration through competing mechanisms. Polacheck WJ, Charest JL, Kamm RD. Proc. Natl. Acad. Sci. USA, 2011. 108 (27):11115-20 Mechanotransduction of fluid stresses governs 3D cell migration. Polacheck WJ, German AE, Mammoto A, Ingber DE, Kamm RD. Proc. Natl. Acad. Sci. USA, 2014. 111 (7):2447-2452 ? 2.5. Invasion and Migration Cell migration into scaffolds under co-culture conditions in a microfluidic platform. Chung S, Sudo R, Mack PJ, Wan CR, Vickerman V, Kamm RD. Lab Chip, 2009. 9 (2):269-275 Concentration gradients in microfluidic 3D matrix cell culture systems. Zervantonakis I, Chung S, Sudo R, Zhang M, Charest J, Kamm R. International Journal of Micro-Nano Scale Transport, 2010. 1 (1):27-36 A novel microfluidic platform for high-resolution imaging of a three-dimensional cell culture under a controlled hypoxic environment. Funamoto K, Zervantonakis IK, Liu Y, Ochs CJ, Kim C, Kamm RD. Lab Chip, 2012. 12 (22):4855-4863 Hydrogels: Extracellular Matrix Heterogeneity Regulates Three-Dimensional Morphologies of Breast Adenocarcinoma Cell Invasion. Shin Y, Kim H, Han S, Won J, Jeong HE, Lee E-S, . . . Chung S. Advanced Healthcare Materials, 2013. 2 (6):920-920 A three-dimensional microfluidic tumor cell migration assay to screen the effect of anti-migratory drugs and interstitial flow. Kalchman J, Fujioka S, Chung S, Kikkawa Y, Mitaka T, Kamm RD, . . . Sudo R. Microfluid. Nanofluid., 2013. 14 (6):969-981 Breast Cancer Cell Invasion into a Three Dimensional Tumor-Stroma Microenvironment. Truong D, Puleo J, Llave A, Mouneimne G, Kamm RD, Nikkhah M. Sci. Rep., 2016. 6:34094 Macrophage-secreted TNFα and TGFβ1 Influence Migration Speed and Persistence of Cancer Cells in 3D Tissue Culture via Independent Pathways. Li R, Hebert JD, Lee TA, Xing H, Boussommier-Calleja A, Hynes RO, . . . Kamm RD. Cancer Res., 2016. 77 (2):279-290 *MBNL1 alternative splicing isoforms play opposing roles in cancer. Tabaglio T, Low DHP, Teo WKL, Goy PA, Cywoniuk P, Wollmann H... Guccione E. Life Science Alliance, Sept 2018 doi:10.26508/lsa.201800157 ? 2.6. Testing New Therapeutic Approaches Engineering a 3D microfluidic culture platform for tumor-treating field application. Pavesi A, Adriani G, Tay A, Warkiani ME, Yeap WH, Wong SC, Kamm RD. Sci. Rep., 2016. 6:26584 *Assessing Therapeutic Efficacy of MEK Inhibition in a KRASG12C-Driven Mouse Model of Lung Cancer. Li S, Liu S, Deng J, Akbay EA, Hai J, Ambrogio C ... Wong KK. Clinical Cancer Research 2018 doi: 10.1158/1078-0432.CCR-17-3438 ↑ Back to the top 3. Immunotherapy *?A 3D microfluidic model for preclinical evaluation of TCR-engineered T cells against solid tumors. Pavesi A, Tan AT, Koh S, Chia A, Colombo M, Antonecchia E, Miccolis C, Ceccarello E, Adriani G, Raimondi MT, Kamm RD, Bertoletti A. JCI Insight. 2017 Jun 15;2(12). pii: 89762. doi: 10.1172/jci.insight.89762. *Ex Vivo Profiling of PD-1 Blockade Using Organotypic Tumor Spheroids. Jenkins RW, Aref AR, Lizotte PH, Ivanova E, Stinson S, Zhou CW, ... Barbie DA. Cancer Discov. 2017 Nov 3. pii: CD-17-0833. doi: 10.1158/2159-8290.CD-17-0833. *CDK4/6 Inhibition Augments Anti-Tumor Immunity by Enhancing T Cell Activation. Deng J, Wang ES, Jenkins RW, Li S, Dries R, Yates K, ... Wong KK. Cancer Discov. 2017 Nov 3. pii: CD-17-0915. doi: 10.1158/2159-8290.CD-17-0915. Characterizing the Role of Monocytes in T Cell Cancer Immunotherapy Using a 3D Microfluidic Model. Lee SWL, Adriani G, Ceccarello E, Pavesi A, Tan AT, Bertoletti A, Kamm RD and Wong SC (2018) Front. Immunol. 9:416. doi: 10.3389/ mmu.2018.00416 *Tumor innate immunity primed by specific interferon-stimulated endogenous retroviruses. Ca?adas I, Thummalapalli R, Kim JW, Kitajima S, Jenkins RW, Christensen CL... Barbie DA. Nature Medicine 23 July 2018 doi.org/10.1038/s41591-018-0116-5 *Molecular recalibration of PD-1+ antigen-specific T cells from blood and liver. Otano I, Escors D, Schurich A, Singh H, Robertson F, Davidson BR... Maini MK. Molecular Therapy (2018), doi: 10.1016/j.ymthe.2018.08.013. *3D microfluidic ex vivo culture of organotypic tumor spheroids to model immune checkpoint blockade. Aref AR, Campisi M, Ivanova E, Portell A, Larios D, Piel BP... Jenkins RW. Lab on a Chip, 2018, DOI: 10.1039/C8LC00322J ↑ Back to the top 4. Neurobiology A high-throughput microfluidic assay to study neurite response to growth factor gradients. Kothapalli CR, van Veen E, de Valence S, Chung S, Zervantonakis IK, Gertler FB, Kamm RD. Lab Chip, 2011. 11 (3):497-507 A microfluidic device to investigate axon targeting by limited numbers of purified cortical projection neuron subtypes. Tharin S, Kothapalli CR, Ozdinler PH, Pasquina L, Chung S, Varner J, . . . Macklis JD. Integr. Biol., 2012. 4 (11):1398-1405 Three-dimensional extracellular matrix-mediated neural stem cell differentiation in a microfluidic device. Han S, Yang K, Shin Y, Lee JS, Kamm RD, Chung S, Cho SW. Lab Chip, 2012. 12 (13):2305-2308 A 3D neurovascular microfluidic model consisting of neurons, astrocytes and cerebral endothelial cells as blood-brain barrier. Adriani G, Ma DL, Pavesi A, Kamm R, Goh ELK. Lab Chip, 2016. 17 (3):448-459 3D self-organized microvascular model of the human blood-brain barrier with endothelial cells, pericytes and astrocytes. Campisi M, Shin YJ, Osaki T, Hajal C, Chiono V, Kamm RD. Biomaterials 2018 https://doi.org/10.1016/j.biomaterials.2018.07.014 ↑ Back to the top 5. Stem Cell Biology 5.1. Differentiation of Embryonic Stem Cells Differentiation of embryonic stem cells into cardiomyocytes in a compliant microfluidic system. Wan CR, Chung S, Kamm RD. Ann. Biomed. Eng., 2011. 39 (6):1840-1847 Simultaneous or Sequential Orthogonal Gradient Formation in a 3D Cell Culture Microfluidic Platform. Uzel SGM, Amadi OC, Pearl TM, Lee RT, So PTC, Kamm RD. Small, 2016. 12 (5):612-622 ? 5.2. Electrical and Mechanical Stimulation of Mesenchymal Stem Cells Controlled electromechanical cell stimulation on-a-chip. Pavesi A, Adriani G, Rasponi M, Zervantonakis IK, Fiore GB, Kamm RD. Sci. Rep., 2015. 5:11800 ↑ Back to the top 6. ?Mechanobiology 6.1. Mechanical stimulation of Cardiac Fibroblasts On-chip assessment of human primary cardiac fibroblasts proliferative responses to uniaxial cyclic mechanical strain. Ugolini GS, Rasponi M, Pavesi A, Santoro R, Kamm R, Fiore GB, . . . Soncini M. Biotechnol. Bioeng., 2016. 113 (4):859-869 ? 6.2. Optically Excitable Motor Units Microfluidic device for the formation of optically excitable, three-dimensional, compartmentalized motor units. Uzel SGM, Platt RJ, Subramanian V, Pearl TM, Rowlands CJ, Chan V, . . . Kamm RD. Science Advances, 2016. 2 (8) ↑ Back to the top ?7. Other Models 7.1. Environmental Assessment *Functional human 3D microvascular networks on a chip to study the procoagulant effects of ambient fine particulate matter. Li Y, Pi QM, Wang PC, Liu LJ, Han ZG, Shao Y, Zhai Y, Zuo ZY, Gong ZY, Yang X, Yang W. RSC Adv., 2017, 7, 56108–56116 *Protein corona of airborne nanoscale PM2.5 induces aberrant proliferation of human lung fibroblasts based on a 3D organotypic culture. Li Y, Wang PC, Hu CL, Wang K, Chang Q, Liu LJ, Han ZG, Shao Y, Zhai Y, Zuo ZY, Gong ZY, Wu Y. Scientific Reports volume 8, Article number: 1939(2018) doi:10.1038/s41598-018-20445-7 ↑ Back to the top 8. Reviews Microfluidic Platforms for Studies of Angiogenesis, Cell Migration, and Cell–Cell Interactions. Chung S, Sudo R, Vickerman V, Zervantonakis IK, Kamm RD. Ann. Biomed. Eng., 2010. 38 (3):1164-1177 Microfluidic devices for studying heterotypic cell-cell interactions and tissue specimen cultures under controlled microenvironments. Zervantonakis IK, Kothapalli CR, Chung S, Sudo R, Kamm RD. Biomicrofluidics, 2011. 5 (1) Microfluidic models of vascular functions. Wong KHK, Chan JM, Kamm RD, Tien J. 2012. 14:205-230 Tumor cell migration in complex microenvironments. Polacheck WJ, Zervantonakis IK, Kamm RD. Cell. Mol. Life Sci., 2013. 70 (8):1335-1356 Microfluidic platforms for mechanobiology. Polacheck WJ, Li R, Uzel SGM, Kamm RD. Lab Chip, 2013. 13 (12):2252-2267 Creating living machines. Kamm RD, Bashir R. Ann. Biomed. Eng., 2014. 42 (2):445-459 In vitro models of the metastatic cascade: from local invasion to extravasation. Bersini S, Jeon JS, Moretti M, Kamm RD. Drug Discov. Today, 2014. 19 (6):735-742 Microfabrication and microfluidics for muscle tissue models. Uzel SGM, Pavesi A, Kamm RD. Progress in Biophysics and Molecular Biology, 2014. 115 (2–3):279-293 Single-Cell Migration in Complex Microenvironments: Mechanics and Signaling Dynamics. Mak M, Spill F, Kamm RD, Zaman MH. J. Biomech. Eng., 2016. 138 (2):021004-021004-8 Impact of the physical microenvironment on tumor progression and metastasis. Spill F, Reynolds DS, Kamm RD, Zaman MH. Curr. Opin. Biotechnol., 2016. 40:41-48 Microfluidics: A New Tool for Modeling Cancer-Immune Interactions. Boussommier-Calleja A, Li R, Chen MB, Wong SC, Kamm RD. Trends in Cancer. 2 (1):6-19 Microfluidic models for adoptive cell-mediated cancer immunotherapies. Adriani G, Pavesi A, Tan AT, Bertoletti A, Thiery JP, Kamm RD. Drug Discov. Today, 2016. 21 (9):1472-1478 M2a macrophages induce contact-dependent dispersion of carcinoma cell aggregates. Adriani G, Bai J, Wong SC, Kamm RD, Thiery JP. Macrophage, 2016. 3:e1222