3D超高分辨率熒光成像顯微系統(tǒng) |
abbelight是一款模塊化的多功能的單分子定位顯微鏡(SMLM)系統(tǒng),它的DASEY技術(shù)能夠極大的提高定位精度的同時,還保持在較小的尺寸。該設(shè)備具有高度靈活性,能夠搭載在絕大多數(shù)的倒置顯微鏡上,并且僅僅需要使用一個C-mount(CCD或CMOS所連接的部位)接口,即可將您的倒置顯微鏡直接升級為超分辨顯微鏡。并且改造過程不會破壞原有顯微鏡系統(tǒng)的光路和功能,不會與其它的顯微鏡改造相沖突。
本設(shè)備既在配置上的選擇也十分靈活。它既可以作為顯微鏡的一個升級配件來改造您的顯微鏡,也擁有完整的超分辨系統(tǒng)。讓用戶在獲得專業(yè)的圖像質(zhì)量的同時,享受到經(jīng)濟合理的超分辨升級方案。
SAFe 180 超分辨模組 |
? 成像模式:PLAM、STORM、smFRET、PAINT、SPT ? 光源模式:Epi、TIRF、HILO ? 超高分辨率:25 nm的XY軸分辨率 ? 超大視野:200 × 200 μm2的視野 ? 全自動化控制? 無需高功率激光光源 ? 可升級SAFe 360 | 大視野3D超分辨模塊 |
SAFe 360 超分辨模組 |
? 具有SAFe 180的所有功能 ? 分辨率:15 nm的XYZ軸分辨率 ? 一次可同時采集1.2 μm深度圖像信息 ? 圖像深度:10 μm ? 實時漂移矯正 ? 四色同時成像 ? 活細胞成像模式 | 高精準3D超分辨顯微成像模塊 |
加裝 | TIRF PALM STORM SPT smFRET ...... | 兼容 | Confocal Spinning-Desk Widefield SIM STED |
Now We See......
肌動蛋白 | 細胞足 | 網(wǎng)格蛋白 | 線粒體 |
3D超分辨成像系統(tǒng)-配套試劑
Smart kit | Compatible dyes |
? 10 doses per box ? 200 µL per dose ? 30 sec prepartion ? 2 months in a fridge ? 2 weeks on sample | ? Phalloidin-AF ® 488, WGA-AF ® 488 ? AF ® 532, CF ® 532, Cy3b ? AF ® 555, CF ® 555, AF ® 568, CF ® 568, Cy5, MemBrite™ 568 ? AF ® 647, CF ® 647, AF ® 680, CF ® 680, MemBrite™ 640 |
3D超分辨成像系統(tǒng)-發(fā)表文獻列表
1.TADs are 3D structural units of higher-order chromosome organization in Drosophila, Science Advances 2018, Article reference DOI: 10.1126/sciadv.aar8082
2.Multicolor localization microscopy by deep learning, ArXiv 2018, Article reference arXiv:1807.01637
3.Primary fibroblasts derived from sporadic amyotrophic lateral sclerosis patients do not show ALS cytological lesions, Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration 2018, Article reference DOI: 10.1080/.2018.1431787
4.Quantitative mapping and minimization of super-resolution optical imaging artifacts, Nature Methods 2018, DOI: 10.1038/nmeth.4605
5.Combining 3D single molecule localization strategies for reproducible bioimaging, BioRxiv 2018, Article reference DOI: 10.1101/385799
6.Aberration-accounting calibration for 3D single-molecule localization microscopy, Optics Letters 2017, Article reference DOI: 10.1364/OL.43.000174
7.Podosome Force Generation Machinery: A Local Balance between Protrusion at the Core and Traction at the Ring, ACS Nano 2017, Article reference DOI: 10.1021/acsnano.7b00622
8.Failure of daptomycin to kill Staphylococcus aureus: impact of bacterial membrane fatty acid composition, Antimicrobial Agents and Chemotherapy 2018, Article reference DOI:10.1128/AAC.00023-18
9.Localized Myosin II Activity Regulates Assembly and Plasticity of the Axon Initial Segment, Neuron 2017, Article reference DOI: 10.1016/j.neuron.2017.12.039
10.Nuclear pore complex plasticity during developmental process as revealed by super-resolution microscopy, Scientific Reports 2017, DOI: 10.1038/s41598-017-15433-2