主要功能

• 成像功能：對 Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等至少 17 種參數(shù)進(jìn)行成像分析。測定調(diào)節(jié)性能量耗散 Y(NPQ)，反映植物光保護(hù)能力，測定非調(diào)節(jié)性能量耗散Y(NO)，反映植物光損傷程度。

• 程序測量功能：可程序測量熒光誘導(dǎo)曲線、快速光曲線和暗弛豫，也可手動測量；在測量過程中能自動分析所有熒光參數(shù)的變化趨勢

• AOI 功能：可在測量前或測量后任意選擇感興趣的區(qū)域（AOI），程序?qū)⒆詣訉x擇的 AOI 的數(shù)據(jù)進(jìn)行變化趨勢分析，并在報(bào)告文件中顯示相關(guān) AOI 的數(shù)據(jù)。所有報(bào)告文件中顯示的數(shù)據(jù)都可導(dǎo)出到 EXCEL 文件中。

• 成像異質(zhì)性分析功能：對任意參數(shù)任意時間的成像，可在圖像上任意選取兩點(diǎn)，軟件自動對兩點(diǎn)間的數(shù)據(jù)進(jìn)行橫向異質(zhì)性分析，并可導(dǎo)出到 EXCEL 文件中。

• 成像數(shù)據(jù)范圍分析功能：對任意參數(shù)任意時間的成像，可分析任意兩個熒光數(shù)值之間有多少個像素點(diǎn)，多少面積（cm²）。

• 突變株篩選功能：可跟據(jù)成像結(jié)果快速篩選光合、產(chǎn)氫/油、抗逆（抗鹽、抗旱、抗病等）等突變株。

• 微藻毒理研究功能：可同時測量 96 個微藻樣品（對照和處理組）的光合活性，軟件自動給出處理組樣品相對于對照組的光合抑制百分比。

• 吸光系數(shù)測量功能：快速測量葉片的吸光系數(shù)。吸光系數(shù)測量光源： 16 個紅光（650 nm）和 16 個近紅外（780 nm）LED，用于測量植物葉片或藻類樣品 PAR 吸光系數(shù)。

測量參數(shù)

Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等。

應(yīng)用領(lǐng)域

• 光合作用研究：可以在*相同的條件下同時對大量樣品進(jìn)行成像

• 植物病理學(xué)：病斑部位（包括肉眼不可見時）成像以及病斑擴(kuò)散的時空動力學(xué)

• 植物脅迫生 理學(xué)：肉眼不可見脅迫損傷的早期檢測

• 遺傳育種：出苗后大規(guī)模快速篩選高光合/抗旱/抗熱/抗凍/抗病等植株

• 突變株篩選：快速篩選模式植物的光合突變株、抗逆突變株、產(chǎn)氫微藻突變株等

• 微藻毒理學(xué)：不同毒物濃度多個重復(fù)的樣品一次測完，軟件自動計(jì)算抑制比率

• 分子生物學(xué)：宏觀水平上檢測樣品的綠色熒光蛋白（GFP）熒光

• 其它多種擴(kuò)展研究

主要技術(shù)參數(shù)

MAXI 探頭（藍(lán)光版）

• 熒光測量光源： 44 個藍(lán)色 LED，450 nm，測量光強(qiáng)度 0.5 μmol m^-2 s^-1，光化光強(qiáng)度  1200 μmol m^-2 s^-1，飽和脈沖強(qiáng)度 2800 μmol m^-2 s^-1。

• 吸光系數(shù)測量光源：16個紅光（650 nm）和 16 個近紅外（780 nm）LED，用于測量樣品 PAR 吸光系數(shù)。

• 成像面積：10×13 cm。

• 光強(qiáng)異質(zhì)性：測量區(qū)域光強(qiáng)異質(zhì)性小于 ±7％。

• 測量參數(shù)：Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等。

MAXI 探頭（紅光版）

• 熒光測量光源： 44 個紅色 LED，620 nm，測量光強(qiáng)度 0.5 μmol m^-2 s^-1，光化光強(qiáng)度 1200 μmol m^-2 s^-1，飽和脈沖強(qiáng)度 2800 μmol m^-2 s^-1。

• 吸光系數(shù)測量光源：16 個紅光（650 nm）和 16 個近紅外（780 nm）LED，用于測量樣品 PAR 吸光系數(shù)。

• 成像面積：10×13 cm。

• 光強(qiáng)異質(zhì)性：測量區(qū)域光強(qiáng)異質(zhì)性小于 ±7％。

• 測量參數(shù)：Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等。

MINI 探頭（藍(lán)光版，MINI 探頭成像方便野外使用）

• 熒光測量光源：12 個藍(lán)色 LED，460 nm，測量光強(qiáng)度 0.5 μmol m^-2 s^-1，光化光強(qiáng)度 2000 μmol m^-2 s^-1，飽和脈沖強(qiáng)度 6000 μmol m^-2 s^-1。

• 吸光系數(shù)測量光源：10 個紅光（650 nm）和 10 個近紅外（780 nm）LED，用于測量樣品 PAR 吸光系數(shù)。

• 信號檢測：1/3” 數(shù)碼相機(jī) CCD，640×480 象素，采集速率 30 幀/秒，帶選擇性鎖相放大器。通過千兆以太網(wǎng)（GigE）與電腦數(shù)據(jù)通訊。

• 成像面積：24×32 mm，6倍放大。

• 測量參數(shù)：Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等。

MINI 探頭（紅光版，MINI 探頭成像方便野外使用）

• 熒光測量光源：12 個紅色 LED，620 nm，測量光強(qiáng)度 0.5 μmol m^-2 s^-1，光化光強(qiáng)度 2000 μmol m^-2 s^-1，飽和脈沖強(qiáng)度 6000 μmol m^-2 s^-1。

• 吸光系數(shù)測量光源：10個紅光（650 nm）和 10 個近紅外（780 nm）LED，用于測量樣品 PAR 吸光系數(shù)。

• 信號檢測：1/3” 數(shù)碼相機(jī)CCD，640×480 象素，采集速率 30 幀/秒，帶選擇性鎖相放大器。通過千兆以太網(wǎng)（GigE）與電腦數(shù)據(jù)通訊。

• 成像面積：24×32 mm，6倍放大。

• 測量參數(shù)：Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等。

選購指南

一、熒光成像 MAXI 版

系統(tǒng)組成：通用型主機(jī)，LED 供電單元， LED 光源，CCD，數(shù)據(jù)線，軟件等。

注意：高等植物或真核藻類測量時選擇藍(lán)光光源（推薦），藍(lán)藻測量請選擇紅光光源。

熒光成像 MAXI 版本

二、熒光成像 MINI 版

系統(tǒng)組成：通用型主機(jī)（下圖中未顯示），LED 光源（如下圖），CCD，數(shù)據(jù)線，軟件等。

注意：高等植物或真核藻類測量時選擇藍(lán)光光源（推薦），藍(lán)藻測量請選擇紅光光源。

熒光成像 MINI 版

三、熒光成像 MICROSCOPY 版

系統(tǒng)組成：通用型主機(jī)，特制顯微鏡，CCD，數(shù)據(jù)線，軟件等。

熒光成像 MICROSCOPY 版

葡萄葉片 Y(NPQ) 成像	葡萄葉片 PS/50 成像結(jié)果	MINI 版本成像結(jié)果
顯微版本成像結(jié)果	軟件成像界面	慢速誘導(dǎo)動力學(xué)曲線

產(chǎn)地：德國 WALZ

參考文獻(xiàn)

數(shù)據(jù)來源：光合作用文獻(xiàn) Endnote 數(shù)據(jù)庫，更新至 2016 年 9 月，文獻(xiàn)數(shù)量超過 6000 篇

原始數(shù)據(jù)來源：Google Scholar

Baek, K., et al. (2016). "DNA-free two-gene knockout in Chlamydomonas reinhardtii via CRISPR-Cas9 ribonucleoproteins." Scientific Reports 6: 30620.

Belbin, F. E., et al. (2016). "Integration of light and circadian signals that regulate chloroplast transcription by a nuclear-encoded sigma factor." New Phytologist: n/a-n/a.

Borlongan, I. A., et al. (2016). "Thermal and PAR effects on the photosynthesis of Eucheuma denticulatum and Kappaphycus striatus (so-called Sacol strain) c*ted in shallow bottom of Bali, Indonesia." Journal of Applied Phycology: 1-10.

Cabral, C., et al. (2016). "Arbuscular mycorrhizal fungi modify nutrient allocation and composition in wheat (Triticum aestivum L.) subjected to heat-stress." Plant and Soil: 1-15.

Chen, D., et al. (2016). "Silicon-moderated K-deficiency-induced leaf chlorosis by decreasing putrescine accumulation in sorghum." Annals of Botany.

Chernysheva, M. G., et al. (2016). "Humic substances alter the uptake and toxicity of nanodiamonds in wheat seedlings." Journal of Soils and Sediments: 1-12.

da Silva, J. M. (2016). "Monitoring Photosynthesis by In Vivo Chlorophyll Fluorescence: Application to High-Throughput Plant Phenotyping."

Gomes, M. P. and P. Juneau (2016). "Oxidative stress in duckweed (Lemna minor L.) induced by glyphosate: Is the mitochondrial electron transport chain a target of this herbicide?" Environmental Pollution.

Gondor, O. K., et al. (2016). "Salicylic Acid and Sodium Salicylate Alleviate Cadmium Toxicity to Different Extents in Maize (Zea mays L.)." PLoS ONE 11(8): e0160157.

H?gele, F., et al. (2016). "Quality Improvement of Fresh-Cut Endive (Cichorium endivia L.) and Recycling of Washing Water by Low-Dose UV-C Irradiation." Food and Bioprocess Technology: 1-12.

Hasan, M. K., et al. (2016). "Glutathione-mediated regulation of nitric oxide, S-nitrosothiol and redox homeostasis confers cadmium tolerance by inducing transcription factors and stress response genes in tomato." Chemosphere 161: 536-545.

Hassler, S., et al. (2016). "Function of the Golgi-located phosphate transporter PHT4;6 is critical for senescence-associated processes in Arabidopsis." Journal of Experimental Botany.

Hu, R., et al. (2016). "Dehydration-responsive features of Atrichum undulatum." Journal of Plant Research: 1-10.

Kakuszi, A., et al. (2016). "Light piping driven photosynthesis in the soil: Low-light adapted active photosynthetic apparatus in the under-soil hypocotyl segments of bean (Phaseolus vulgaris)." Journal of Photochemistry and Photobiology B: Biology 161: 422-429.

Li, L., et al. (2016). "Characterization of a novel β-barrel protein (AtOM47) from the mitochondrial outer membrane of Arabidopsis thaliana." Journal of Experimental Botany.

Li, L., et al. (2016). "Physiological and biochemical characterization of sheepgrass (Leymus chinensis) reveals insights into photosynthetic apparatus coping with low-phosphate stress conditions." Journal of Plant Biology 59(4): 336-346.

Li, M.-Q., et al. (2016). "Melatonin mediates selenium-induced tolerance to cadmium stress in tomato plants." Journal of pineal research: n/a-n/a.

Li, Q., et al. (2016). "Functional conservation and divergence of GmCHLI genes in polyploid soybean." The Plant Journal: n/a-n/a.

Li, X., et al. (2016). "Melatonin enhances cold tolerance in drought primed wild type and abscisic acid-deficient mutant barley." Journal of pineal research: n/a-n/a.

Li, Z.-X., et al. (2016). "Developmental changes in carbon and nitrogen metabolism affect tea quality in different leaf position." Plant Physiology and Biochemistry 106: 327-335.

Li, Z., et al. (2016). "Evolution of an atypical de-epoxidase for photoprotection in the green lineage." Nature Plants 2: 16140.

Lin, Y.-C., et al. (2016). "Arabidopsis PHOSPHATIDYLGLYCEROPHOSPHATE PHOSPHATASE1 (PGPP1) Involved in Phosphatidylglycerol Biosynthesis and Photosynthetic Function." The Plant Journal: n/a-n/a.

Liu, D., et al. (2016). "Peracetic acid is a suitable disinfectant for recirculating fish-microalgae integrated multi-trophic aquaculture systems." Aquaculture Reports 4: 136-142.

Loro, G., et al. (2016). "Chloroplast-specific in vivo Ca2+ imaging using Yellow Cameleon fluorescent protein sensors reveals organelle-autonomous Ca2+ signatures in the stroma." Plant Physiology.

Nielsen, S. L., et al. (2016). "Copper in the sediment: a major stressor for eelgrass, Zostera marina L." Hydrobiologia: 1-13.

Pell, L., et al. (2016). "Mild disintegration methods of microalgae–bacteria flocs from wastewater treatment." Journal of Applied Phycology: 1-9.

Pompeiano, A., et al. (2016). "Allocation pattern, ion partitioning, and chlorophyll a fluorescence in Arundo donax L. in responses to salinity stress." Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology: 1-10.

Rios, J. A., et al. (2016). "Effects of blast on components of wheat physiology and grain yield as influenced by fungicide treatment and host resistance." Plant Pathology: n/a-n/a.

Schmidt, C., et al. (2016). "Retention of high thermal tolerance in the invasive foraminifera Amphistegina lobifera from the Eastern Mediterranean and the Gulf of Aqaba." Marine Biology 163(11): 228.

Shu, S., et al. (2016). "The role of 24-epibrassinolide in the regulation of photosynthetic characteristics and nitrogen metabolism of tomato seedlings under a combined low temperature and weak light stress." Plant Physiology and Biochemistry 107: 344-353.

Tatagiba, S. D., et al. (2016). "Magnesium decreases leaf scald symptoms on rice leaves and preserves their photosynthetic performance." Plant Physiology and Biochemistry 108: 49-56.

von Sydow, L., et al. (2016). "The PsbY protein of Arabidopsis Photosystem II is important for the redox control of Cytochrome b559." Biochimica et Biophysica Acta (BBA) - Bioenergetics.

Wang, M., et al. (2016). "Submergence Induced Changes of Molecular Species in Membrane Lipids in Arabidopsis thaliana." Plant Diversity.

Wang, P., et al. (2016). "Rapid in-season detection of herbicide resistant Alopecurus myosuroides using a fluorescence imaging sensor." Crop Protection 89: 170-177.

Wang, Y., et al. (2016). "Different responses of photosystem II and antioxidants to drought stress in two contrasting populations of Sour jujube from the Loess Plateau, China." Ecological research: 1-15.

Wang, Y., et al. (2016). "Chlorophyll Fluorescence in Leaves of Ficus tikoua Under Arsenic Stress." Bulletin of Environmental Contamination and Toxicology 97(4): 576-581.

Watanabe, Y., et al. (2016). "Photosynthetic responses of Pyropia yezoensis f. narawaensis (Bangiales, Rhodophyta) to a thermal and PAR gradient vary with the life-history stage." Phycologia 55(6): 665-672.

Xu, Q., et al. (2017). "Volatile organic compounds released from Microcystis flos-aquae under nitrogen sources and their toxic effects on Chlorella vulgaris." Ecotoxicology and Environmental Safety 135: 191-200.

Xu, W., et al. (2016). "Melatonin enhances thermotolerance by promoting cellular protein protection in tomato plants." Journal of pineal research: n/a-n/a.

Zhang, X., et al. (2016). "The miR396b of Poncirus trifoliata functions in cold tolerance by regulating ACC oxidase gene expression and modulating ethylene-polyamine homeostasis." Plant and Cell Physiology.

Zhang, X., et al. (2016). "ER-localized adenine nucleotide transporter ER-ANT1: an integrator of energy and stress signaling in rice." Plant Molecular Biology: 1-15.

Zhang, Y., et al. (2016). "Photosynthetic capacity of the capsule wall and its contribution to carbon fixation and seed yield in castor (Ricinus communis L.)." Acta Physiologiae Plantarum 38(10): 245.